Industry: food & beverage

Ensuring that water, juice, beer, wine and other beverages, dietary supplements and food ingredients, contain low levels of contaminants and are safe to eat, but include the stated amounts of nutrients, is of primary concern to ensure a healthy life for people.
International recommendations on handling of food and analysis of their constituents have been set by WHO and additional guidelines how to analyse food substances are provided in the Food Chemicals Codex assembled by USP. Many regional authorities, including EFSA and national food government agencies are working with WHO and USP to enforce these recommendations and mitigate food fraud. Diduco has contributed by showing that analysis of anions in drinking water according to the requirements set by EPA method 300.1 which also satisfy the demands by corresponding ISO and ASTM methods, and that these analysis can be performed with a flexible equipment to which a setup for suppressed ion chromatography have been connected.

Applications

Tartrate in dietary supplements
ion chromatography

Analysis of dietary supplement capsules with carnitine using a generic method for counterions

Oral L-carnitine is a dietary supplement that has been shown to reduce fatigue in elderly people with low muscular endurance, but its effects on athletes during physical activity are not well-supported. Although this endogenous compound is well-known for its involvement in the mitochondrial oxidation of long-chain fatty acids, no scientific evidence has been found that carnitine in isolation enhances fat-burning.

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Amino acids by gradient HILIC-ELSD and TSKgel Amide-80
HILIC

Separation of selected amino acids

Amino acids are the fundamental building blocks of all proteins and therefore essential constituents of many nutritional supplements and can also be present in pharmaceutical formulations. Due to the highly hydrophilic nature and zwitterionic charge at neutral pH of many amino acids and their structurally related degradation products, hydrophilic interaction chromatography is an attractive mode of separation that require no derivatization, while evaporative light scattering enables rather sensitive detection of these poorly light-absorbing compounds.

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Simple carbohydrates by HILIC-ELSD and Luna Omega Sugar
HILIC

Separation of simple sugars and carbohydrates

Simple carbohydrates are frequent ingredients in drinks, food, dietary supplements and pharmaceutical formulations. One approach to their analysis is hydrophilic interaction chromatography combined with evaporative light scattering detection, although this might be challenging due to the possibility of reducing sugars to mutarotate and thereby change their conformation into other anomers with different retention.

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Cannabinoids in hemp using Raptor ARC-18
reversed-phase

Analysis of cannabinoids in dried industrial hemp after methanol extraction

The legislation in many countries allow cultivation of industrial hemp provided that the strains do not produce high amounts of tetrahydrocannabinol (THC) and other psychoactive cannabinoids classified as narcotic substances. These strains of cannabis sativa are instead typically rich in compounds such as cannabidiol (CBD) and cannabidiolic acid (CBDA).

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Pharamaceutical counterions with XAMS suppressor and ASUREX-A200
ion chromatography

Generic method for isocratic separation of fifteen common pharmaceutical counterions

Many medicines and dietary supplements are bases that carry a positive charge, and these small molecules are consequently accompanied by negatively charged counterions. Such anionic excipients may influence the properties of the pharmaceutical formulation and can also influence the effect of the active ingredient since different ion pairs can have different dissolution rates or abilities to penetrate cell walls.

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Pharamaceutical counterions with XAMS suppressor and ASUREX-A200
ion chromatography

Generic method for isocratic separation of ten common pharmaceutical counterions

Many medicines and dietary supplements are bases that carry a positive charge, and these small molecules are consequently accompanied by negatively charged counterions. Such anionic excipients may influence the properties of the pharmaceutical formulation and can also influence the effect of the active ingredient since different ion pairs can have different dissolution rates or abilities to penetrate cell walls.

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I’ll be happy to answer any questions you might have regarding our products and services.

Tobias Jonsson
CEO, PhD in chemistry
Mobile: +46 705783490
E-mail: tobias@diduco.com

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