This procedure allows quantification of the seven common inorganic anions fluoride, chloride, nitrite, bromide, nitrate, phosphate and sulfate. This application was set up on a flexible modular stainless steel based HPLC system, equipped with the Xenoic® XAMS membrane suppressor and the Xenoic® ASUREX automatic regenerator, and could meet the requirements of the EPA method. This procedure was updated to version 300.1A in 1997.
Quantitative analysis of anions in bottled water according to the method US EPA 300.0A using a Shodex IC SI-90 4E column (250×4 mm) and an eluent containing 1.7 mM Na₂CO₃ and 1.8 mM NaHCO₃ in ultra-pure water pumped at 1.0 mL/min at 25 °C. Background reduced to ~13 µS/cm by XAMS suppressor with ASUREX-A100 automatic regenerator. Eluent pumping and conductivity detection by a passivated Hitachi LaChrom Elite system. Injection of 50 µL sample. Linear six-point calibration curves 0.2-7 mg/L (ppm) gave the following concentrations; Cl¯ 0.41±0.17 mg/L and SO₄²¯ 2.6±0.1 mg/L (95% confidence interval). F¯ was estimated to 0.1 mg/L, but this was below the calibration range.
Diduco is a leading provider of professional services in high performance liquid chromatography and manufacturer of the Xenoic® XAMS chemical suppressor and ASUREX automatic regenerator for ion chromatography.
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