Analysis of cannabinoids in dried industrial hemp after methanol extraction

Analysis of cannabidiolic acid (CBDA) and cannabidiol (CBD) in dried industrial hemp after methanol extraction. Reversed phase HPLC separation on Raptor ARC-18 core-shell column (150×4.6 mm, 2.7 µm) protected by a matching guard column, using an eluent containing 75% acetonitrile and 25% aqueous ammonium formate (1.25 mM) plus 0.1% formic acid as additive, pumped at 1.5 mL/min at 30 °C. A Shimadzu system with modules SCL-10Avp, 2x LC-10ADvp, DGU-14A, SIL-10ADvp, CTO-10ASvp, SPD-M10Avp were used and detection was performed at 210 nm. Injection of 5 µL of dried hemp (0.5 g) that had been vortexed, sonicated and centrifuged in methanol, and then diluted in methanol and filtered using Separa filter vials (PTFE, 0.2 µm). Quantification using linear seven-point calibration curves prepared from certified reference material standards diluted in methanol.