This potency analysis exemplifies quality control of industrial hemp using classic reversed-phase separation with ultra-violet (UV) light absorption detection. Dry plant material samples were thoroughly extracted by methanol, to allow accurate quantification of a range of cannabinoids in different types of samples, without interference from related substances.
Analysis of cannabinoids in dried industrial hemp after methanol extraction
The legislation in many countries allow cultivation of industrial hemp provided that the strains do not produce high amounts of tetrahydrocannabinol (THC) and other psychoactive cannabinoids classified as narcotic substances. These strains of cannabis sativa are instead typically rich in compounds such as cannabidiol (CBD) and cannabidiolic acid (CBDA).
Analysis of cannabidiolic acid (CBDA) and cannabidiol (CBD) in dried industrial hemp after methanol extraction. Reversed phase HPLC separation on Raptor ARC-18 core-shell column (150×4.6 mm, 2.7 µm) protected by a matching guard column, using an eluent containing 75% acetonitrile and 25% aqueous ammonium formate (1.25 mM) plus 0.1% formic acid as additive, pumped at 1.5 mL/min at 30 °C. A Shimadzu system with modules SCL-10Avp, 2x LC-10ADvp, DGU-14A, SIL-10ADvp, CTO-10ASvp, SPD-M10Avp were used and detection was performed at 210 nm. Injection of 5 µL of dried hemp (0.5 g) that had been vortexed, sonicated and centrifuged in methanol, and then diluted in methanol and filtered using Separa filter vials (PTFE, 0.2 µm). Quantification using linear seven-point calibration curves prepared from certified reference material standards diluted in methanol.
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