HILIC
Separation of selected amino acids
Amino acids are the fundamental building blocks of all proteins and therefore essential constituents of many nutritional supplements and can also be present in pharmaceutical formulations. Due to the highly hydrophilic nature and zwitterionic charge at neutral pH of many amino acids and their structurally related degradation products, hydrophilic interaction chromatography is an attractive mode of separation that require no derivatization, while evaporative light scattering enables rather sensitive detection of these poorly light-absorbing compounds.
