The green revolution currently taking place in development of sustainable chemicals and plastics, relies on optimizing new efficient reaction paths. These synthesis procedures are often catalysed by enzymes in several steps, making it crucial to perform reaction monitoring to simultaneously quantify starting materials, intermediates, and end products.
When tracing the fate of components during development of multi-step chemical reactions, the flexible analysis offered by reversed-phase HPLC is typically a reliable choice. During the early stages of exploring new strains of enzymes and new reaction conditions, analysis methods allowing discovery of unpredicted by-products and intermediates may be crucial for a successful project outcome. Diduco deliver input on method setup and provide open-access equipment for scientists at innovative companies or research groups contributing to advancements in this field.