This application shows baseline separation of the nucleosides, uridine, adenosine, and cytidine, together with their corresponding nucleobases, thymine, uracil, adenine, and cytosine, on a zwitterionic column with a bonded phosphorylcholine stationary phase under HILIC conditions that do not compromise the solubility of the compounds.
Isocratic separation of four nucleosides and their nucleobases on a Merck SeQuant ZIC-cHILIC column (100×4.6 mm) using an eluent containing 5 mM ammonium acetate in 80% acetonitrile/water, pumped at 0.5 mL/min at 22 °C. Eluent pumping, injection and detection by an Agilent HP 1050 system with quaternary pump, autosampler, and diode array detector (at 254 nm) controlled by ChemStation A10.01 software. Injection of 5 µL standards diluted in eluent to concentrations of 5-10 mg/L. Toluene was used as an approximate marker for the void volume.
Diduco is a leading provider of professional services in high performance liquid chromatography and manufacturer of the Xenoic® XAMS chemical suppressor and ASUREX automatic regenerator for ion chromatography.
Diduco AB
Tvistevägen 48C
90736 Umeå
Sweden